The new medium MDSS2N, free of any animal protein supports cell growth and production of various viruses
Identifieur interne : 000270 ( France/Analysis ); précédent : 000269; suivant : 000271The new medium MDSS2N, free of any animal protein supports cell growth and production of various viruses
Auteurs : O. W. Merten [France] ; H. Kallel [Tunisie] ; Jean-Claude Manuguerra [France] ; P. Tardy-Panit [France] ; R. Crainic [France] ; Francis Delpeyroux [France] ; S. Van Der Werf [France] ; P. Perrin [France]Source :
- Cytotechnology [ 0920-9069 ] ; 1999.
Abstract
The development of media free of serum and animal or human proteins is of utmost importance for increasing the safety of biologicals produced for therapy and vaccination. In order to reduce the risk of contamination, we have modified the serum free medium MDSS2, a very efficient serum free medium for the production of various biologicals including experimental vaccines using different cell lines (Merten et al., 1994), by replacing the animal derived products by plant extracts. The new serum and animal protein free medium (MDSS2N) can be efficiently used for biomass production of various cell lines. These cells grow equally well or better in this new serum-free medium than in the old formulation (MDSS2):* BHK-21/BRS cells, adapted to MDSS2N, showed an overall specific growth rate of 0.0197 h-1 (mu_max = 0.0510+/-0.0058 h-1), whereas those cultivated in MDSS2 grew with an average specific growth rate of 0.0179 h-1 (mu_max = 0.0305+/-0.0177 h-1).* Vero cells grew with an average specific growth rate of 0.0159 h-1 and 0.0153 h-1 in MDSS2 and MDSS2N, respectively. Very similar growth rates were obtained in microcarrier cultures in stirred tank reactors: the specific growth rates were 0.0161 h-1 and 0.0166 h-1 for MDSS2 and MDSS2N cultures, respectively.* For MDCK cells, when cultured on microcarriers in bioreactors, a higher average specific growth rate was observed in MDSS2N than in MDSS2; values of 0.0248 h-1 and 0.0168 h-1, respectively, were obtained.The capacity of MDSS2N to support the production of different viruses was equally evaluated and it could be established that for certain viruses there are no or insignificant differences between MDSS2N and MDSS2 (influenza and polio virus), whereas, the production of rabies virus is somewhat reduced in MDSS2N when compared to MDSS2. The use of MDSS2N for cell culture and the production of various viruses is discussed.
Url:
DOI: 10.1023/A:1008021317639
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: 000296
- to stream Hal, to step Curation: 000296
- to stream Hal, to step Checkpoint: 000337
- to stream Main, to step Merge: 001A10
- to stream Main, to step Curation: 001967
- to stream Main, to step Exploration: 001967
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Links to Exploration step
Hal:pasteur-02044584Le document en format XML
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<author><name sortKey="Crainic, R" sort="Crainic, R" uniqKey="Crainic R" first="R." last="Crainic">R. Crainic</name>
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<country>France</country>
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<author><name sortKey="Delpeyroux, Francis" sort="Delpeyroux, Francis" uniqKey="Delpeyroux F" first="Francis" last="Delpeyroux">Francis Delpeyroux</name>
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<country>France</country>
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<author><name sortKey="Van Der Werf, S" sort="Van Der Werf, S" uniqKey="Van Der Werf S" first="S." last="Van Der Werf">S. Van Der Werf</name>
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<orgName>Université Paris Diderot - Paris 7</orgName>
<orgName type="acronym">UPD7</orgName>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine>
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<author><name sortKey="Perrin, P" sort="Perrin, P" uniqKey="Perrin P" first="P." last="Perrin">P. Perrin</name>
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<desc> <address> <addrLine>25-28 rue du Dr Roux - 75724 Paris Cedex 15</addrLine>
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<country>France</country>
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</analytic>
<idno type="DOI">10.1023/A:1008021317639</idno>
<series><title level="j">Cytotechnology</title>
<idno type="ISSN">0920-9069</idno>
<imprint><date type="datePub">1999</date>
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<front><div type="abstract" xml:lang="en"> <p>The development of media free of serum and animal or human proteins is of utmost importance for increasing the safety of biologicals produced for therapy and vaccination. In order to reduce the risk of contamination, we have modified the serum free medium MDSS2, a very efficient serum free medium for the production of various biologicals including experimental vaccines using different cell lines (Merten et al., 1994), by replacing the animal derived products by plant extracts. The new serum and animal protein free medium (MDSS2N) can be efficiently used for biomass production of various cell lines. These cells grow equally well or better in this new serum-free medium than in the old formulation (MDSS2):* BHK-21/BRS cells, adapted to MDSS2N, showed an overall specific growth rate of 0.0197 h-1 (mu_max = 0.0510+/-0.0058 h-1), whereas those cultivated in MDSS2 grew with an average specific growth rate of 0.0179 h-1 (mu_max = 0.0305+/-0.0177 h-1).* Vero cells grew with an average specific growth rate of 0.0159 h-1 and 0.0153 h-1 in MDSS2 and MDSS2N, respectively. Very similar growth rates were obtained in microcarrier cultures in stirred tank reactors: the specific growth rates were 0.0161 h-1 and 0.0166 h-1 for MDSS2 and MDSS2N cultures, respectively.* For MDCK cells, when cultured on microcarriers in bioreactors, a higher average specific growth rate was observed in MDSS2N than in MDSS2; values of 0.0248 h-1 and 0.0168 h-1, respectively, were obtained.The capacity of MDSS2N to support the production of different viruses was equally evaluated and it could be established that for certain viruses there are no or insignificant differences between MDSS2N and MDSS2 (influenza and polio virus), whereas, the production of rabies virus is somewhat reduced in MDSS2N when compared to MDSS2. The use of MDSS2N for cell culture and the production of various viruses is discussed.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
<li>Tunisie</li>
</country>
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<tree><country name="France"><noRegion><name sortKey="Merten, O W" sort="Merten, O W" uniqKey="Merten O" first="O. W." last="Merten">O. W. Merten</name>
</noRegion>
<name sortKey="Crainic, R" sort="Crainic, R" uniqKey="Crainic R" first="R." last="Crainic">R. Crainic</name>
<name sortKey="Delpeyroux, Francis" sort="Delpeyroux, Francis" uniqKey="Delpeyroux F" first="Francis" last="Delpeyroux">Francis Delpeyroux</name>
<name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name>
<name sortKey="Perrin, P" sort="Perrin, P" uniqKey="Perrin P" first="P." last="Perrin">P. Perrin</name>
<name sortKey="Tardy Panit, P" sort="Tardy Panit, P" uniqKey="Tardy Panit P" first="P" last="Tardy-Panit">P. Tardy-Panit</name>
<name sortKey="Van Der Werf, S" sort="Van Der Werf, S" uniqKey="Van Der Werf S" first="S." last="Van Der Werf">S. Van Der Werf</name>
</country>
<country name="Tunisie"><noRegion><name sortKey="Kallel, H" sort="Kallel, H" uniqKey="Kallel H" first="H." last="Kallel">H. Kallel</name>
</noRegion>
</country>
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</affiliations>
</record>
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